Figure 4.

Impact of the Roux-en-Y gastric bypass surgery on portal vein and peripheral plasma metabolic profiles of Zucker obese rats

PCA scores plots (a, b, c) of ¹H NMR spectral data obtained from peripheral (dot) and portal vein (triangle) samples from RYGB (blue), sham-BWM (green) and sham-obese (red) Zucker rats at 4-week post-operation. A heatmap (d) of significantly changed metabolites between RYGB and Sham-obese or Sham-BWM in systemic (s) and portal vein blood plasma (p). Correlation coefficient (r) shown in the heatmap were obtained from OPLS-DA models. Positive (red) or negative (blue) correlation coefficient values indicate higher or lower concentrations of the metabolites in RYGB or RYGB portal vein compared with either of the sham groups or RYGB peripheral plasma. *, + and # indicate significant levels of metabolite differences between the groups at p < .05, p < .01 and p < .001, respectively, using two-tailed heteroscedastic t-test with Benjamini-Hochberg correction. ^a Metabolites with a p = .06 post BH correction.