Table 1.
Summary of the main reports performing DNA-EV analyses for liquid biopsy tests.
| References | Body fluid | Tumor type | Method | EV type | DNAase | Yield | DNA size | Biomarkers/platform |
|---|---|---|---|---|---|---|---|---|
| Krug et al. (2017) | Plasma | NSCLC | Exo-Elution™ Plus extraction kit | Small and large EVs | No | ND | ND | EGFR T790M by NGS |
| Castellanos-Rizaldos et al. (2018) | Plasma | NSCLC | Exo-Elution™ Plus extraction kit | Small and large EVs | No | ND | ND | EGFR mutations by NGS |
| Allenson et al. (2017) | Plasma | Pancreatic cancer | Ultracentrifugation | Small EVs | No | ND | ND | KRAS mutations by ddPCR |
| Yang et al. (2017) | Serum | Pancreatic cancer | Ultracentrifugation | Small EVs | No | ND | ND | KRAS and TP53 mutations by ddPCR |
| San Lucas et al. (2016) | Pleural fluid | Pancreatic cancer | Ultracentrifugation | Small EVs | No | ND | >10kb | Tumor profiling by Illumina Hiseq2500 |
| Figueroa et al. (2017) | CSF | Glioblastoma | Ultracentrifugation | Small EVs | Yes | ND | ND | EGFRvIII mutation by QPCR |
| Garcia-Silva et al. (2019) | Lymphatic exudate | Melanoma | Exo-Elution™ Plus extraction kit | Small and large EVs | No | ND | ND | BRAF V600E by QPCR |
| Hur et al. (2019) | BALF | NSCLC | Ultracentrifugation | Small EVs | No | ND | ND | EGFR mutations by QPCR |
| Song et al. (2019) | Pleural effusion fluid | NSCLC | Exo-Elution™ Plus extraction kit | Small and large EVs | No | ND | ND | Targeted-NGS |
| Lee et al. (2018) | Urine | Bladder cancer | ExoQuick-TC | Small EVs | No | ND | ND | Mutation analysis by target capture sequencing /CNV by whole genome sequencing |
CFS, cerebrospinal fluid; BALF, bronchoalveolar lavage fluid; NSCLC, non-small cell lung cancer; PDAC, pancreatic ductal adenocarcinoma; ND, not determined.