| Reagent | Supplier |
|---|---|
| DMEM, high glucose | Gibco, Fisher Scientific (11-965-092) |
| optiMEM | Gibco, Thermo Fisher Scientific (11058021) |
| fetal bovine serum | Neuromics (FBS001) |
| penicillin/streptomycin (10,000 units/mL) | Gibco, Fisher Scientific (15-140-122) |
| puromycin | Gibco, Thermo Fisher Scientific (A1113803) |
| trypsin (+phenol-red) | Gibco, Thermo Fisher Scientific (25300062) |
| trypsin (no phenol-red) | Gibco, Thermo Fisher Scientific (15400054) |