Skip to main content
. Author manuscript; available in PMC: 2021 Jul 1.
Published in final edited form as: Kidney Int. 2020 Feb 22;98(1):76–87. doi: 10.1016/j.kint.2020.01.036

Table 1.

Primers used in quantitative reverse transcription polymerase chain reactions to amplify mouse cDNAs based on published GenBank sequences. Annealing temperatures used for each primer are also provided.

Primers Sequence (Sense/Antisense) Annealing Temp (°C)
mouse TNF-α 5’-TACTGAACTTCGGGGTGATTGGTCC-3’ 65 °C
5’-CAGCCTTGTCCCTTGAAGAGAACC-3’
mouse MCP-1 5’-ACCTGCTGCTACTCATTCAC-3’ 60 °C
5’-TTGAGGTGGTTGTGGAAAAG-3’
mouse MIP-2 5’-CCAAGGGTTGACTTCAAGAAC-3’ 60 °C
5’-AGCGAGGCACATCAGGTACG-3’
mouse KC 5’-CAATGAGCTGCGCTGTCAGTG-3’ 60 °C
5’-CTTGGGGACACCTTTTAGCATC-3’
mouse IL-6 5’-CCGGAGAGGAGACTTCACAG-3’ 62 °C
5’-GGAAATTGGGGTAGGAAGGA-3’
mouse ICAM-1 5’-TGTTTCCTGCCTCTGAAGC-3’ 60 °C
5’-CTTCGTTTGTGATCCTCCG-3’
mouse NGAL 5’-CACCACGGACTACAACCAGTTCGC-3’ 66 °C
5’-TCAGTTGTCAATGCATTGGTCGGTG-3’
GAPDH 5’-ACCACAGTCCATGCCATCAC-3’ 65 °C
5’-CACCACCCTGTTGCTGTAGCC-3’