Figure 6.

Effects of the five compounds found in GFE on tight junction protein expressions in DEP-stimulated HaCaT cells. (a) Cells were pretreated with geniposide, geniposidic acid, chlorogenic acid, genipin, or gardenoside (1, 10, 50 μM) for 24 h. Cell viabilities were assessed using an XTT kit. (b) Cells were pretreated with geniposide, geniposidic acid, chlorogenic acid, genipin, or gardenoside (50 μM) for 1 h and then stimulated with DEP (100 μg/ml) for 24 h. The expressions of occludin and claudin were determined by western blot. (c) Densitometry analysis of occludin and claudin expression. Data are presented as mean ± SD (n = 3). ^#P < 0.05 vs. normal controls (NC), ^∗P < 0.05 vs. DEP treated cells.