Figure 6.

Bixin upregulates Nrf2 signals and PPARα involved in HFD-induced hepatic steatosis. (a) Liver tissue sections from different groups of Nrf2 WT and Nrf2 KO mice were subjected to Oil Red O staining; black arrow indicates the infiltration of inflammatory cells. (n = 10; scale bar = 100 μm. Representative images from each group are shown). (b) Liver tissue lysates from each group were subjected to immunoblot analyses with the indicated antibodies. Representative blots of three independent samples from each group were shown; quantification of relative protein expression was determined; results are expressed as the means ± SD (n = 10; ^∗p < 0.05, Ctrl vs. treatment groups; ^#p < 0.05, HFD group vs. HFD+bixin group).