Expression of TNFs vital to secondary lymphoid tissue formation in vertebrates. (A) Expression of TNFs in previously published genomes and transcriptomes. (B) Expression of TNFs in O-MALT sequenced from mouse PP, turkey CT, African lungfish LA, and trout ILT. *, an amino acid percentage identity with human ≥30%; +, amino an acid percentage identity with human between 30 and 20%; —, amino acid percentage identity with human of <20%. The red boxes denote important TNFs for secondary lymphoid tissue development that were absent in searched genomes.