Figure 2.

Mutational analyses reveal critical host and viral determinants of ACE-SARS-CoV-2 RBD interaction

(A) Binding interface of the RBD and ACE2 with potential critical ACE2 residues of interest highlighted. (B and C) A bioreporter assay (B) and immunoblot analysis (C) were performed on cells co-transfected with RBD-LgBiT and SmBiT-ACE2 mutant constructs. IVIS imaging of bioreporter assay results are shown at the bottom of (B). (D) Binding interface of the RBD and ACE2 with potential critical RBD residues of interest highlighted. (E and F) Biosensor (BS) assay (E) and immunoblot analysis (F) were performed on cells co-transfected with RBD mutant-LgBiT constructs and SmBiT-ACE2 constructs. IVIS imaging of bioluminescence is shown at bottom of (E). Immunoblot analyses were performed to confirm proper expression of the constructs.