Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Feb 5;16:851–865. doi: 10.2147/IJN.S296513

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2021 Sears et al.

This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).

PMC Copyright notice

TAgNPs are more cytotoxic to TNBC than to non-malignant mammary epithelial cells. (A) Relative viability of MCF-10A and MDA-MB-231 after 72-hour treatment with TAgNPs (0–50 µg/mL). Viability was assessed by MTT assay. Data is representative of at least 3 independent experiments. Statistical analysis was performed by two-way ANOVA followed by post hoc Tukey’s test. Statistical differences are indicated (*p<0.05; **p<0.01). (B) The dose per unit area in comparison to the concentration of TAgNPs was calculated for various well sizes and is shown in tabular form. (C) MDA-MB-231 and (D) MCF-10A cells were treated with 12.5 µg/mL TAgNPs for 24 hours. Cells were co-stained with propidium iodide (PI) and annexin V (AnnV), and then evaluated by flow cytometry. The percentages of cells characterized as viable (lower-left quadrant), early apoptotic (lower-right quadrant), late apoptotic (upper-right quadrant) and necrotic (upper-left quadrant) are shown with each quadrant. The presented data are representative of duplicate independent experiments.