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. 2021 Feb 5;13:1099–1111. doi: 10.2147/CMAR.S278023

Figure 5.

Figure 5

The celastrol/circ_SLIT3 axis regulated HCC progression in vitro by miR-223-3p. (A) miR-223-3p expression by qRT-PCR in celastrol (Cela)-treated Huh7 and Hep3B cells transfected with Vector, circ_SLIT3, circ_SLIT3+miR-NC mimic or circ_SLIT3+miR-223-3p mimic. Huh7 and Hep3B cells were transfected with Vector, circ_SLIT3, circ_SLIT3+miR-NC mimic or circ_SLIT3+miR-223-3p mimic for 24 h and then treated with 1 µM of celastrol (Cela) for 48 h, followed by the determination of cell colony formation by colony formation assay (B), cell proliferation by MTS assay (C and D), Ki67 level by Western blot (E), cell migration and invasion by transwell assay (F and G), cell apoptosis by flow cytometry (H), the levels of Bcl-2 and Bax by Western blot (I and J). Vector: negative control plasmid, circ_SLIT3: circ_SLIT3 overexpressing plasmid. n = 3; data were presented as mean ± SD; *P < 0.05, **P < 0.01 or ***P < 0.001 vs Vector, circ_SLIT3+miR-NC, Control, Cela+Vector or Cela+circ_SLIT3+miR-NC.