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. 2021 Jan 7;11:622748. doi: 10.3389/fpls.2020.622748

FIGURE 2.

FIGURE 2

Impact of short-term heat stress on pollen-pistil interactions. (A) Pollen viability (%) and pollen germination (%) of non-stressed, and heat-stressed (40°C for 4 h) pollen. (B) Seed yield, mg produced from the fertilization of pistils pollinated during reciprocal crosses. (C) Aniline blue staining of reciprocal crosses (NS♀× NS♂, NS♀× HS♂, HS♀× NS♂ and HS♀× HS♂) to observe pollen-pistil interactions. (D) Siliques, seed filling of siliques, total seeds produced by the reciprocal crosses. Data are represented as the mean of three replicates ± SEM (standard error of the mean). The asterisk (*) represents a significant difference between heat stress treatment and non-stressed control (∗∗∗P ≤ 0.001).