Suppl. Fig. 1. PTSD-induction paradigm experimental outline. A. On day 1, animals performed an open field test to screen for locomotor or trait-anxiety behavior which could interfere with behavioral analyses. On day 5 and 6, animals were exposed to the PTSD-induction, which involved two sessions of inescapable electric foot shock: a relatively intense “Trauma” session on day 5, followed by a relatively less intense “Trigger” session on day 6 (B). Animals subsequently performed a series of behavioral tests for PTSD-like behaviors (dark/light transfer for risk assessment, marble burying for compulsive behavior, acoustic startle for hyperarousal, and home cage activity for disrupted sleep cycle) (A,C). The 20% of animals displaying the most “PTSD-like” behavior in each test (reduced risk assessment, increased marble burying, reduced pre-pulse inhibition and latency to peak startle amplitude, and increased light phase activity) were awarded points for that test (C). Animals scoring more than 4 points total were designated as “PTSD-like” while animals scoring no points were designated as “Resilient.” On day 22 animals performed a 25 min restraint stress test for corticosterone response (A). Finally, on day 31 animals were sacrificed via live decapitation (A).