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. 2021 Jan 27;8:629835. doi: 10.3389/fchem.2020.629835

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Copyright © 2021 Prajapati, Karan, Khezerlou and DeCoster.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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CRL2303 glioma cells response to 20 μg/ml CuHARS treatment in presence of inflammatory stimulations (100 ng/ml of TNF+ 5 μg/ml of LPS). (A) NO concentration in the cells due to the treatment showing significant difference in NO release from CRL2303 cells treated with CuHARS compared to cells treated with inflammatory stimulus alone. The negative controls treated with LNAME (LN), a NOS inhibitor, showed significant inhibition in NO release. (B) Cell metabolism due to the treatment showing inhibition of cell metabolism for CuHARS treated glioma cells including in the presence of inflammatory stimuli (as in Panel A). Data represent average of three experiments (N = 3) with triplicated wells (n = 3). The error bars represent SEM values, “***” represents p < 0.001, and “*” represents p < 0.05.