FIGURE 6.

Normal brain endothelial cell (BMVECs) response to 20 μg/ml CuHARS treatment in presence of three different concentrations (10, 25 and 50 µM) of CysNO. (A) NO concentration in the BMVECs due to the treatment showing significant increase in NO release from cells treated with CuHARS compared to cells treated with CysNO alone. (B) Cell metabolism due to the treatment showing increase in cellular metabolism for CuHARS treated cells compared to when treated with CysNO alone. Data represent average of three experiments (N = 3) with triplicated wells (n = 3). The error bars represent SEM values, “***” represents p < 0.001, “**” represents p < 0.01 and “*” represents p < 0.05.