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. 2021 Jan 27;8:629835. doi: 10.3389/fchem.2020.629835

FIGURE 7.

FIGURE 7

Normal brain endothelial cell (BMVECs) response to 20 μg/ml CuHARS treatment in the presence of inflammatory stimulations (100 ng/ml of TNF + 5 μg/ml of LPS). (A) NO concentration in the cells due to the treatment showing inhibition in NO release in both 48- and 96-h time point in BMVECs treated with CuHARS compared to cells treated with inflammatory stimulus alone. The negative controls treated with LNAME, an NOS inhibitor, showed significant inhibition in NO release. (B) Cell metabolism due to the treatment showing significant increase in cell metabolism in BMVECs treated with inflammatory stimulus compared to the controls and slight inhibition due to CuHARS in the cells treated with the combination of CuHARS and inflammatory stimulus. Data represent average of three experiments (N = 3) with triplicated wells (n = 3). The error bars represent SEM values, “***” represents p < 0.001, “**” represent p < 0.01 and “*” represents p < 0.05.