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. 2021 Jan 27;11:605618. doi: 10.3389/fphar.2020.605618

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Copyright © 2021 Ji, Du, Wang, Wu, Chen, Hua and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Adult CRD precipitated the visceral hypersensitivity in rats experiencing neonatal CRD. (A) Rats undergoing neonatal CRDs on postnatal days 8, 10, and 12, and adult CRD in week 8. The behavioral testing was conducted in weeks 8–10. (B) Pain threshold was measured immediately after adult CRD (n = 6 rats each, F_3,20 = 25.83, p < 0.001). (C) Pain threshold was measured 2 h after adult CRD (n = 6 rats each, F_3,20 = 16.34, p < 0.001). Significance was assessed by ordinary one-way ANOVA with post hoc comparisons between groups. The data are expressed as the mean ± SEM. **p < 0.01.