Figure 3.

Mcl-1 deficiency impaired DNA damage repair with elevation of oxidative stress. Two-week-old male Mcl-1^fl/fl and Mcl-1^Δhep mice injected with PBS or DEN (20 mg/kg BW) were sacrificed at 2, 4 and 6 weeks of age. (A,B) Representative images of liver sections stained with anti-γ-H2AX (A) or anti-4-HNE (B) antibody (left). The scale bar is 50 µm. γ-H2AX-positive and 4-HNE-positive hepatocyte ratios were quantified and are shown as percentages (right) (n ≥ 4 per group, mean ± SEM, Mann–Whitney test, *p < 0.05). (C) Representative immunoblots of HO-1 in the liver of six-week-old mice (left), and expression levels of HO-1 by real- time PCR in the liver of six-week-old mice (right) (n = 8 per group, mean ± SEM, Mann–Whitney test, *p < 0.05). Full-length blots/gels are presented in Supplementary Fig. 2.