Fig. 2.
MSC-AS1 knockdown inhibited cell proliferation and induced TMZ sensitivity and cell apoptosis in TMZ-resistant glioma cells. LN229/TR and SHG-44/TR cells were transfected with si-NC or si-MSC-AS1-1. a and b MSC-AS1 expression was measured by qRT-PCR. c Cell viability and IC50 value of TMZ were evaluated using CCK-8 assay. d and e The number of cell colony and cell apoptosis rate were determined by colony formation assay and flow cytometry assay, respectively. f The protein levels of MCL-1, MRP-1, cyclinD1, Bax, P-PI3K, P-AKT, and caspase-3 were detected by western blot. *P < 0.05