Fig. 6.

Silencing miR-373-3p reversed the inhibitory effect of CPEB4 knockdown on TMZ resistance in TMZ-resistant glioma cells. LN229/TR and SHG-44/TR cells were transfected with si-NC, si-CPEB4, si-CPEB4 + inhibitor NC, or si-CPEB4 + miR-373-3p inhibitor. a and b The mRNA and protein levels of CPEB4 were detected by qRT-PCR and western blot, respectively. c Cell viability and IC₅₀ value of TMZ were examined by CCK-8 assay. d and e The number of cell colony and cell apoptosis rate were determined by colony formation assay and flow cytometry assay, respectively. f and g The levels of MCL-1, MRP-1, cyclinD1, Bax, and caspase-3 were measured by western blot. *P < 0.05