Figure 2.

Morphology of BM-MSCs expanded at P5 stage. (a1, a2, h1, h2) BM-MSCs cultured in DMEM-KO + 10% FBS at a seeding density of 1000 cells/cm² (a1, a2) and 5000 cells/cm² (h1, h2).(a1) Microphotographs at 10–20% confluency, (a2): 80–90% confluency, h1: 50–55% confluency and h2: 80–90%confluency. Similarly (b1, b2, i1, i2)- BM-MSCs cultured in RoosterNourish. (c1, c2, j1, j2)- BM-MSCs cultured in RoosterNourish-MSC XF. d1, d2, k1, k2- BM-MSCs cultured in StemMACS-MSC XF. (e1, e2, i1, i2) BM-MSCs cultured in StemXVivo serum-free media. (f1, f2, m1, m2) BM-MSCs cultured in PLTMax human platelet lysate. g1, g2, n1, n2- BM-MSCs cultured in MSC NutriStem XF. (b1, b2, c1, c2, d1, d2, e1, e2, f1, f2, g1, g2)- MSC at a seeding density of 1000 cells/cm² and (i1, i2, j1, j2, k1, k2, l1, l2, m1, m2, n1, n2) 5000 cells/cm². (b1, c1, d1, f1, g1) Microphotographs at 20–25% confluency. (b2, c2, d2, f2, g2): 80–90% confluency. (i1, j1, k1, m1, n1): 50–55% confluency and (i2, j2, k2, m2, n2): 80–90% confluency. (e1 and e2): Microphotographs at 10–15% confluency and 40–45% confluency. l1 and l2: Microphotographs at 20–25% confluency and 40–50% confluency. Experiments were performed in duplicates and the representative images are shown. The images were captured at 10X magnification with scale bar of ∼100 μm.