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. 2021 Feb 9;11:3403. doi: 10.1038/s41598-021-83088-1

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Comparison of yield and viability of BM-MSCs in six different low serum/SFM/XFM and control medium expanded at P4 (a & b) and P5 (c & d). BM-MSCs were cultured in respective medium with two different seeding densities (1000 and 5000 cells/cm²). Each of the medium tested supported the growth of MSCs at P4 in both the seeding densities and the cell yield and viability was comparable with control medium (a & b). Except StemXVivo SFM, all other medium tested supported the growth of MSCs in subsequent passage (P5). The yield and viability of BM-MSCs cultured in low serum/SFM/XFM was comparable with control medium at P5. (c & d). Experiments were performed in duplicates. The data is represented as mean ± SD, *p < 0.05.