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. 2021 Feb 9;11:3403. doi: 10.1038/s41598-021-83088-1

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Population doublings and doubling time of BM-MSCs grown in low serum/SFM/XFM and control medium. (a) Comparison of PDT and CPDs of BM-MSCs cultured in low serum/SFM/XFM and control medium at P4 and P5 at seeding density of 1000 cells/cm² (b) 5000 cells/cm². MSCs cultured in SFM/XFM at 1000 cells/cm² had higher CPDs than MSCs cultured at 5000 cells/cm². BM-MSCs cultured in low serum/SFM/XFM showed varied PDT ranging from 21.89 ± 0.5 h to 45.7 ± 1.66 h except for StemXVivo SFM. c) The average cell diameter of BM-MSCs cultured in different low serum/SFM/XFM and control medium analyzed through Vi-CELL XR Cell Viability Analyzer. The average diameter of the BM-MSCs cultured in low serum/SFM/XFM ranged from 16.02 µm ± 0.16 to 19.2 µm ± 0.22. The data is represented as mean ± SD (n = 2*p < 0.05). SCM: Serum containing medium, RN: RoosterNourish, RN-XF: RoosterNourish-MSC XF, SM-XF: StemMACS-MSC XF, PM-hPL: PLTMax human platelet lysate, NS-XF: MSC NutriStem XF, SV-SFM: StemXVivo SFM.