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. 2021 Feb 9;11:3403. doi: 10.1038/s41598-021-83088-1

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Colony forming ability, potency and immunosuppressive potential of BM-MSCs cultured in SFM/XFM and control medium. (a) Representative photograph of CFU-F of BM-MSCs grown in low serum/ SFM/XFM and control medium. (b) Bar graph showing the number of colonies of BM-MSCs cultured in low serum/SFM/XFM and control medium. The data is represented as mean ± SD (n = 2; *p < 0.05). (c) Cryopreserved cells were seeded into T-75 flasks post revival and grown in respective media for 72 h and the amount of VEGF secreted was determined by ELISA. Results are expressed in concentration (ng/ml/million cells) as mean of duplicate experiment. The amount of VEGF secretion was higher in BM-MSCs cultured in PLTMax hPL when compared to other media and control medium. (d) Bar graph shows the percentage proliferation of PHA stimulated T cell blasts in MSC: PHA activated PBMC co-cultures cultured in SFM/XFM and control medium. The proliferation index in co-cultures is relative to proliferation in PHA blasts (cultured in the absence of MSCs) which was considered as 100% (e) To determine IDO enzyme activity, the culture supernatant of MSC: PHA activated PBMC co-cultures of different media groups was assayed by spectrophotometric detection of the kynurenine concentration (tryptophan metabolite which is a product of IDO catabolism) and is represented as Kynuerurine units (μM). IDO secretion was higher in co-cultures cultured in SFM/XFM when compared to serum-containing medium.