. 2021 Feb 9;11:3403. doi: 10.1038/s41598-021-83088-1

Table 2.

List of primers used for RT-PCR analysis.

Gene	Primer sequence	Product length
GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase)	Forward: TGGTATCGTGGAAGGACTCATGAC Reverse: ATGCCAGTGAGCTTCCCGTTCAGC	189 bp
PPARG (Peroxisome Proliferator Activated Receptor Gamma)	Forward: CCATGCTGTTATGGGTGAAA Reverse: TCAAAGGAGTGGGAGTGGTC	192 bp
FABP4 (Fatty Acid Binding Protein 4)	Forward: ACCTTAGATGGGGGTGTCCTGGT Reverse: CGCCTTTCATGACGCATTCCACC	112 bp
LPL (Lipoprotein Lipase)	Forward: ATGGAGAGCAAAGCCCTGCTC Reverse: GTTAGGTCCAGCTGGATCGAG	564 bp
RUNX2 (RUNX Family Transcription Factor 2)	Forward: CCACTGAACCAAAAAGAAATCCC Reverse: GAAAACAACACATAGCCAAACGC	129 bp
PARC (Secreted Protein Acidic And Cysteine Rich)	Forward: TTCAGCCAGGAAGGCCAAAA Reverse: GGGGAGGGTGAAGAAAAGGA	146 bp
TNFRSF11B (TNF Receptor Superfamily Member 11b)	Forward: GTGGAATAGATGTTACCCTGTGTG Reverse: TGCTCGAAGGTGAGGTTAGC	298 bp
SOX9 (SRY-Box Transcription Factor 9)	Forward: GGCAAGCTCTGGAGACTTCTG Reverse: CCCGTTCTTCACCGACTTCC	138 bp
COL1A1 (Collagen Type I Alpha 1 Chain)	Forward: AGGGCTCCAACGAGATCGAGATCCG Reverse: TACAGGAAGCAGACAGGGCCAACGTCG	223 bp
COL10A1 (Collagen Type X Alpha 1 Chain)	Forward: AGCCAGGGTTGCCAGGACCA Reverse: TTTTCCCACTCCAGGAGGGC	386 bp

Gene

Primer sequence

Product length

GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase)

Forward: TGGTATCGTGGAAGGACTCATGAC

Reverse: ATGCCAGTGAGCTTCCCGTTCAGC

189 bp

PPARG

(Peroxisome Proliferator Activated Receptor Gamma)

Forward: CCATGCTGTTATGGGTGAAA

Reverse: TCAAAGGAGTGGGAGTGGTC

192 bp

FABP4

(Fatty Acid Binding Protein 4)

Forward: ACCTTAGATGGGGGTGTCCTGGT

Reverse: CGCCTTTCATGACGCATTCCACC

112 bp

LPL

(Lipoprotein Lipase)

Forward: ATGGAGAGCAAAGCCCTGCTC

Reverse: GTTAGGTCCAGCTGGATCGAG

564 bp

RUNX2

(RUNX Family Transcription Factor 2)

Forward: CCACTGAACCAAAAAGAAATCCC

Reverse: GAAAACAACACATAGCCAAACGC

129 bp

PARC

(Secreted Protein Acidic And Cysteine Rich)

Forward: TTCAGCCAGGAAGGCCAAAA

Reverse: GGGGAGGGTGAAGAAAAGGA

146 bp

TNFRSF11B

(TNF Receptor Superfamily Member 11b)

Forward: GTGGAATAGATGTTACCCTGTGTG

Reverse: TGCTCGAAGGTGAGGTTAGC

298 bp

SOX9

(SRY-Box Transcription Factor 9)

Forward: GGCAAGCTCTGGAGACTTCTG

Reverse: CCCGTTCTTCACCGACTTCC

138 bp

COL1A1

(Collagen Type I Alpha 1 Chain)

Forward: AGGGCTCCAACGAGATCGAGATCCG

Reverse: TACAGGAAGCAGACAGGGCCAACGTCG

223 bp

COL10A1

(Collagen Type X Alpha 1 Chain)

Forward: AGCCAGGGTTGCCAGGACCA

Reverse: TTTTCCCACTCCAGGAGGGC

386 bp