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. 2021 Feb 9;11:3438. doi: 10.1038/s41598-021-82901-1

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Astrocytic hApoEε4 potentiates MC1 pathology produced by tau oligomers. Scale bar of representative images represents 50 µM. High-content imaging and associated quantification represents data from one independent experiment. (a) Immunocytochemistry of co-cultures five days after addition with 100 nM of tau monomers or tau oligomers. Oligomers were added five days after transduction with the P_GFAP construct of interest. Cells were fixed and stained with MC1 to measure tau pathology and MAP2 to label neurons. Hoechst stain was used to label nuclei. Arrowheads highlight examples of MC1-positive cell bodies and neurites. Top panel depicts images without MAP2 imaging overlay to aid in visualization. (b) High-content imaging to quantify MAP2-positive Hoechst-labeled nuclei (n = 4 wells/group). (c,d) Quantification of MC1 intensity (c) and MC1 area (d) in MAP2-labeled cell bodies (n = 4 wells/group). Data were normalized to the oligomer-treated P_GFAP::EV group (n = 4 wells/group). NS not significant, *p < 0.05, **p < 0.01, Tukey multiple comparisons test following one-way ANOVA.