Fig. 5. PCGF2-PRC1 is the most abundant PRC1 complex in ESC.

a A schematic illustrating the central canonical and variant PRC1 complexes. b Representative images showing a single Z-slice from ESCs expressing HaloTag fusions of PRC1 subunits labelled with JF₅₄₉. Scale bar = 5 μm. Imaging was performed in n = 2 biological replicates. c A box plot showing estimates of the number of protein molecules per cell, measured by in-gel fluorescence (green), and corresponding measurements of relative JF₅₄₉ signal for PRC1 subunits (blue). Measured fluorescence intensities have been converted into numbers of molecules by normalisation to the mean number of RING1B molecules per cell. Box plots indicate measurements for n > 35 cells (JF₅₄₉ signal) or n = 3 independent biological replicates of in-gel quantifications (In-gel fluorescence). The means for each replicate of in-gel fluorescence measurements are represented by black dots. Boxes represent the interquartile range (IQR), the middle line corresponds to the median, and whiskers extend to the largest and smallest values no more than 1.5 x IQR from the box. Values outside of this range are not plotted, but are included in all analyses. Source data are provided as a Source Data file.