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. 2021 Feb 9;12:887. doi: 10.1038/s41467-021-21130-6

Fig. 9. The C-terminal RAWUL domain of PCGF1 is required for stable chromatin binding.

Fig. 9

a A schematic illustrating the PCGF1 constructs used for exogenous expression. The RING (aa 47–86, red) and RAWUL domains (aa 190–253, green) are indicated. b A box plot indicating the percentage of each PCGF1 fusion molecule (FL full length) in the bound state. Box plots represent measurements from n = 90 movies from 3 experiments each. Indicated p-values were calculated using a two-tailed Student’s t test. Source data are provided as a Source Data file. c. A box plot indicating the fraction each PCGF1 fusion molecule exhibiting stable binding. Stable binding of PCGF1 1–129 was not measurable (NM). Box plots represent measurements from n = 16 movies from 2 experiments each. The indicated p-value was calculated using a two-tailed Student’s t test. Source data are provided as a Source Data file. In b–c, boxes represent the interquartile range (IQR), the middle line corresponds to the median, and whiskers extend to the largest and smallest values no more than 1.5 x IQR from the box. Values outside of this range are not plotted, but are included in all analyses.