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. 2021 Feb 9;11:3233. doi: 10.1038/s41598-021-82457-0

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Analysis of the specificity of PZP-purified antibodies for their respective antigens using ELISA. PZP-purified antibodies (orange bars) were incubated in wells of microplates coated with the antigens or glycoconjugates similar to the antigens, and the reactivity of each antibody to the antigens was compared with that of the original hybridoma culture supernatant (blue bars). The following main PZP elution fractions were analyzed for each antibody: PA5, 100 mM PB-eluted fraction; PA4.2 and PA7, 200 mM PB-eluted fraction; FR9, 500 mM PB-eluted fraction. Error bars, mean ± S.D. Gb3 Gb3Cer, Gb4 Gb4Cer, Gg3 Gg3Cer, Gg4 Gg4Cer, LN LacNAcCerA, 6SLN α2,6-sialyl LacNAcCerA, 3SLN α2,3-sialyl LacNAcCerA, as-fetuin asialo-fetuin. The oligosaccharide structures of the glycoconjugates used in this analysis are shown in Supplementary Table S1.