Table 3.

Amounts of PZP-purified antibodies in elution fractions.

Clone ID	Culture sup	100 mM	200 mM	500 mM	100–500 mM total
(a) Total amount of antibody in each fraction (μg)
PA4.2	271.5	5.6	122.2	71.1	198.9
PA7	151.9	21.4	21.5	5.5	48.4
FR9	40.3	1.1	10.6	10.4	22.1
MOPC104E	60.8	40.9	10.4	7.1	58.4

Clone ID	100 mM	200 mM	500 mM	100–500 mM total
(b) Recovery rate of antibody in each fraction (%)
PA4.2	2.1	45.0	26.2	73.3
PA7	14.1	14.1	3.6	31.9
FR9	2.6	26.4	25.7	54.8
MOPC104E	67.2	17.1	11.7	96.1

Each antibody in hybridoma culture supernatant or ascites was purified using PZPs as described in the “Methods”, and antibodies adsorbed to PZPs were sequentially eluted using 100, 200, and 500 mM PB (pH 8.0). The total amount of each antibody (a) and the recovery rate from the original sample (b) in each elution fraction is summarized. SDS-PAGE and immunoblotting results for the antibodies in each fraction are shown in Supplementary Figs. S1 and S2.