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. 2021 Jan 27;12:617269. doi: 10.3389/fmicb.2021.617269

Figure 2.

Figure 2

Comparison of viable cell and spore counts produced by three different C. botulinum Group II strains (Beluga, Eklund 17B and FT10F) grown in three challenged media: standard tryptone-peptone-glucose-yeast extract (TPGY) broth, Clostridium perfringens sporulation medium Duncan-Strong (DS), and the newly described biphasic cooked meat medium-TPGY (CMM-TPGY) broth. Total viable cell and spore enumeration (A) and spore heating assays (B) were performed directly after inoculating the fresh media, and subsequently at day 1, day 7, and day 14 post inoculation. Biphasic CMM-TPGY medium promoted most efficiently bacterial growth (A) and sporulation (B) of all three tested C. botulinum Group II strains. The experiment was performed in three parallel biological replicates. The error bars represent the maximum and minimum values of three replicates. *Statistically significant (p<0.05) difference in cell or spore concentration between different media.