FIGURE 2.

The process of implementing beneficial genetic variation identified in CWR in breeding. (A) Expected breeding timeline for different breeding strategies leveraging beneficial genetic variation that was already targeted in CWR. The given time frames are for annual crop breeding without the use of accelerating conditions such as greenhouse or winter nursery. (B) A conceptual pipeline for leveraging genetic variation identified in CWR in breeding, from sampling design and collection, followed with a common garden experiment for phenotyping, analysis of genomic, phenomic, and environmental data to target the causative mutation or tightly linked polymorphism. Once the trait was targeted gnomically, implementation into breeding material could be conducted through direct genome editing (CRISPR) or introgression.