Fig. 2.

Immunofluorescent staining of ZBTB20 overexpression transfected cells. No Flag-tagged signal (yellow) could be detected in the wild-type HHL7 cell line, while strong Flag-tagged signals were detected in cells transfected with ZBTB20 isoforms 1, isoform 2 and OFP, under the same excitation parameters. Strong centralised CTNNB1 signals (red) were detected in both ZBTB20 isoforms 1 and 2 overexpression transfected cells, while only very weak CTNNB1 signals were detected in wild-type HHL7 and OFP control cell lines, under the same excitation parameters. Nuclei of cells were stained with DAPI (blue). Merged image shown in far-right panels. Scale bars, 10 μm. HHL, immortalized human hepatic cell line.