Fig. 5.

ZBTB20 acts as a transcriptional repressor for PPARG expression. (A) Schematic diagram for the PPARG gene locus and the promoter sequence used to construct pGL3-PPARG. (B) Luciferase promoter analyses showing both ZBTB20 isoforms 1 and 2 overexpressing cells were able to decrease PPARG promoter activities by 47% and 45%, respectively, compared with orange fluorescent protein control vector transfected cells. HHL7 wild-type cells (HHL7 WT) were also included for comparison. ∗∗∗, p <0.0005. (C) Summary diagram showing the role of ZBTB20 in suppressing PPARG expression and subsequently, allowing the activation of the canonical WNT/CTNNB1 signalling pathway and its downstream target genes.