Figure 4.

Example of NICHE-seq, assessing the cellular composition of defined niches (215). (A) Two-photon laser scanning microscopy (TPLSM) images of naïve inguinal lymph nodes from PA-GFP host mice before and after photoactivation (green). In red, adoptively transferred cells and cyan marks the T-cell area and the B follicles, respectively. (B) Expression profile from photoactivated B follicles (cyan) or T-cell areas (red). (C) Relative enrichment of different T-cell types [(B), CD4, CD8^low, CD8^high and CD8 Activated] in each subregion. *p < 0.05.