Figure 1.

Increased YTHDF1 expression is associated with CRC

(A) Expression levels of YTHDF1 from 50 colon tumor tissues and their matched normal colon tissues were detected by qRT-PCR. (B) YTHDF1 protein expression levels were detected from eight CRC tumors and their matched normal colon tissues by western blot. β-Actin was a loading control. (C) Representative immunohistochemical staining of YTHDF1 protein expression levels from colon tumor tissues and their matched normal colon tissues. (D) mRNA expression levels of YTHDF1 in normal colon epithelial cells and five CRC cell lines were detected by qRT-PCR. (E and F) DLD-1 (E) and LoVo (F) cells were transfected with control or YTHDF1 overexpression plasmid for 48 h, followed by cisplatin treatments at the indicated concentrations. Cell viability was determined by MTT and clonogenic assays. Columns include mean of three independent experiments; data are presented as mean ± SD. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001.