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. 2021 Jan 27;11:559810. doi: 10.3389/fimmu.2020.559810

Figure 1.

Figure 1

Localization of CD3+ T cells in the perilesional cortices. Representative images of brain sections from CCI mice stained for anti-CD3e (T lymphocytes; white) and anti-GFAP (astrocytes; red), 30 dpi (A, B). The lesion edges in each section are marked with a segmented yellow line. T cells are present within the lesion (star in A and B), in the perilesional cortex (box in A and B, and panel C) and in the corpus callosum (yellow arrow heads in A). CD3+ cells were also observed in the striatum (white arrow in A) and in the thalamus (white arrow head in A). Both scattered cells and presumably-encephalitogenic clusters of T cells (panel D) were found within the parenchyma. Panels (C) represent a magnification of the area depicted within the white boxes in A. Panels (D, E) represent a magnification of the areas depicted within the white box in B. CD3e: white; GFAP: red; DAPI: blue. (A and B, scale bar = 500 µm; C-E, scale bar = 50 µm).