Figure 2.

T cell brain infiltration is confined to the perilesional cortices, 30 dpi. Box plot representing the number of infiltrating T cells, defined by expression of TCRβ (A) and stacked bargram representing the percentage of CD4+ and CD8+ T cells (B) in the brain of WT and TG mice, as analyzed in the perilesional and contralateral cortices (ipsi and contra, respectively; WT ipsi, n = 12; WT contra, n = 7; TG ipsi, n = 10; TG contra, n = 7), or in intact cortices from respective naïve mice (WT naïve, n = 5, TG naïve, n = 9). Independently from the genotype, a significant infiltration of TCRβ+ T cells was observed in the perilesional areas but not in the contralateral hemispheres (comparable to naïve non-injured brains). The majority of brain-infiltrating T cells presented a CD8 phenotype. In the TG CCI mice, there was a significant skew of CD4/CD8 ratio towards CD8+ T cells. Table (C) summarizes the results of the statistical analysis in T cell counts between the experimental groups. In (A) boxes represent the 25–75% value range, including the median value, indicated with the line. Whiskers represent 1.5x standard deviation (SD). □ indicates the mean value. In the stacked bargram, data are presented as mean ± standard error of the mean (s.e.m.). A binomial negative regression or a linear mixed model was applied to assess statistical differences in the counts of total T cells. The Kruskal Wallis test or the paired samples Wilcoxon signed ranked test was used for the analysis of CD4 and CD8 frequency distribution. ^φp < 0.05 and ^φφφp < 0.001 vs. TG ipsi. *p < 0.05 and ***p < 0.001 vs. WT ipsi. In all tests, Bonferroni correction was used to adjust p-values in multiple comparisons.