Figure 4.

Analysis of CD69 and CD44 T cell activation and memory markers in CD4+ and CD8+ subpopulations. Pseudocolor dot plots (A, B) represent gated subpopulations CD69 vs. CD44 of CD8+ and CD4+, respectively. Bargrams in (C, D) show respectively the counts and frequencies of CD8+ T cell subpopulations, as analyzed in the perilesional cortices of WT and TG mice. No significant differences in CD8+ subpopulations were found between genotypes. In CD4+ subpopulation, instead, we observed a significant reduction in the counts of CD44^hiCD69+ and CD44^hiCD69- subpopulations (E), in K14-VEGFR3-Ig compared to WT mice. However, no differences were observed in the different subpopulation frequencies (F). Data are presented as median ± SD. A binomial negative regression was applied to assess statistical differences in the counts of total T cells between WT ipsi and TG ipsi. The Kruskal Wallis test was used for the analysis of frequency distribution. ^#p < 0.05; *p < 0.05 vs. WT ipsi.