Figure 7.

Peripheral immune response in the spleen. The percentages of T cells in the spleen of WT naïve and CCI mice and of TG naïve and CCI mice are presented in the box plot in panel (A). Stacked bargrams in (B) represent the relative percentages of CD4 and CD8 in T cell population, in WT and K14-VEGFR3-Ig mice. K14-VEGFR3-Ig mice present a drastic reduction of T cells compared to WT littermates, due to a decrease in CD8+ T cell frequency. (C, D) Representative pseudocolor dot plots and gating strategies for CD4+ and CD8+ T cell subpopulation analysis, respectively. Bargrams in (E, F) show respectively the frequencies of CD4+ and CD8+ T cell subpopulations, as analyzed in WT and TG mice. Significant differences in the frequencies of both CD4+ and CD8+ subpopulations have been observed. The Kruskal Wallis test or the paired samples Wilcoxon signed ranked test was used for the analysis of frequency distribution. ^¤¤p < 0.01 and ^¤¤¤p <0.001 vs. TG CCI. **p < 0.01 and ***p < 0.001 vs. WT naïve. In all tests, Bonferroni correction was used to adjust p-values in multiple comparison. For box plot and stacked bargram explanation, refer to the legend of Figure 3 .