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. Author manuscript; available in PMC: 2021 Feb 10.
Published in final edited form as: J Mol Biol. 2020 Dec 3;433(2):166733. doi: 10.1016/j.jmb.2020.166733

Figure 1.

Figure 1.

Pol ι interacts with the deubiquitinating enzyme, USP7. (a) Select proteins identified by GeLC-MS/MS analysis of proteins co-immunoprecipitated with FLAG Pol ι from 293T whole cell lysates (WCL). (b) Coimmunoprecipitated proteins and WCL prepared similarly to (a) were immunoblotted as indicated. (c) Endogenous Pol ι was immunoprecipitated from HEK293T whole cell lysate and the eluent and input immunoblotted as indicated. * = non-specific band (d) His Pol ι (1.5 µg) was incubated with equimolar quantities of GST (0.5 µg) or GST-tagged USP7 (2.3 µg). GST was captured on glutathione sepharose beads, washed, and eluted. Protein were separated by gel electrophoresis and proteins stained with colloidal Coomassie blue (shown in grayscale). I = 10% input, FT = 10% flow through, E = 50% eluent.