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. Author manuscript; available in PMC: 2021 Feb 10.
Published in final edited form as: J Mol Biol. 2020 Dec 3;433(2):166733. doi: 10.1016/j.jmb.2020.166733

Figure 3.

Figure 3.

Pol ι associates with the TRAF and UBL1-2 domains of USP7. (a) Schematic illustrating USP7 truncation mutants employed in this figure. (b) Immunoprecipitation of the indicated USP7 truncations from HEK293T cells co-expressing WT FLAG Pol ι. Eluent and WCL (input) were immunoblotted as indicated. * = Myc antibody proteins. (c) WT FLAG Pol ι was co-expressed in 293T cells with WT Myc USP7, or a mutant lacking 19 aa from the C-terminal tail (Δ 19 aa Tail). WCL was then prepared and immunoblotted as indicated. (d) Immunoprecipitation of the indicated USP7 truncations from 293T cells co-expressing WT FLAG Pol ι. Eluent and WCL (input) were immunoblotted as indicated. * = Myc antibody proteins.