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. Author manuscript; available in PMC: 2021 Feb 10.
Published in final edited form as: J Mol Biol. 2020 Dec 3;433(2):166733. doi: 10.1016/j.jmb.2020.166733

Figure 4.

Figure 4.

The USP7 TRAF domain interacts with the Pol ι C terminus. (a) Schematics of Pol ι truncation mutants used in this figure. The numbers above the schematics indicate the location of serine residues that are part of P/A/ExxS motifs. The numbers in red correspond to serine residues mutated in b. Pol ι truncation mutants were immunoprecipitated from cells co-expressing TRAF Myc USP7 and the eluent and WCL (input) immunoblotted as indicated. (b) WT or mutant Pol ι was immunoprecipitated from 293T cells co-expressing WT or DW164AA Myc TRAF USP7. Eluent and WCL (input) were immunoblotted as indicated. (c) ITC profile of binding between Pol ι peptide 573–584 and recombinant TRAF. (d) WT or S580A FLAG Pol ι was co-expressed in HEK293T cells with WT or DW164AA Myc USP7. Whole cell lysates were immunoblotted as indicated.