Figure 3.

Social hierarchy in weanling mice can be established using the untrained tube test Figure S1

(A) Summary of the social ranks of the weanling mice as determined by the untrained tube test performed two times per day over three days (N = 12 cages).

(B) Percentage of each hierarchy relationship observed in weanling mice (N = 12 cages).

(C) Comparison of assay time across four trials for the 3- and 8-week-old mice (pair 1-2: p = 0.186, pair 2-3: p = 0.370, pair 3-4: p = 0.561, pair 1-3: p = 0.259, pair 2-4: p = 0.267, pair 1-4: p = 0.057, unpaired t test or Mann-Whitney test, N = 12 pairs).

(D) Comparison of assay variation across four trials for the 3- and 8-week-old mice (pair 1-2: p = 0.369, pair 2-3: p > 0.999, pair 3-4: p = 0.883, pair 1-3: p = 0.626, pair 2-4: p > 0.999, pair 1-4: p = 0.784, Mann-Whitney test, N = 12 pairs).

(E) Summary of social ranks tested every week from 3-week-old to 8-week-old (N = 12 cages). (F) Correlation of social ranks for the 3- and 8-week-old mice (Pearson Correlation, N = 12 cages).

(G) Total time of dominant and subordinate mice on the platform in platform competition assay (p = 0.011, Mann-Whitney test, N = 12 cages).

(H) Correlation of social ranks defined by tube test and platform competition assay (Pearson Correlation, N = 12 cages). Mean ± S.E.M.

See also Figure S1.