Fig. 1.

MiR-147 expression across different cell lines, tissue, and cells. a Transcript level of miR-147 in various human cell lines (kidney-derived cell lines including HEK293T and HK2; endothelial cell line, HMEC1; colon-derived cell lines including T84, Caco2, and HL60; lung-derived cell lines including A549 and Calu3; peripheral blood–derived cell line, THP1). Expression level was normalized to U6 snoRNA. b Transcript level of miR-147 in human primary cells including human cardiomyocytes (HCM), human pulmonary alveolar epithelial cells (HPAEpiC), and human monocyte-derived macrophages (MDM). Expression level was normalized to U6 snoRNA. c Screening of miR-147 expression in different organs collected from C57BL/6J mice. Expression level was normalized to U6 snoRNA. d Transcript level of miR-147 in murine primary epithelial cells derived from airway, colon, and renal tubules. Expression level was normalized to U6 snoRNA. e Transcript level of miR-147 in immune cells such as lymphocytes, monocytes, and neutrophils isolated from the peripheral blood of C57BL/6J mice. Expression level was normalized to U6 snoRNA. f Transcript level of miR-147 in Th0, Th1, Th2, Th17, and Treg cells differentiated from naïve CD4 T cells of C57BL/6J mice. Data was normalized to Th0. All graphs represent mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 by one-way ANOVA with Bonferroni’s multiple comparisons.