Fig. 3.

Strategy for the generation of miR-147 floxed mice and miR-147^−/− mice. a Schematic illustration for the generation of miR-147 conditional knockout mice. b Southern blotting with Neo and 3′ probes to confirm targeted ES cells. I-IC7 (lane 1, 2) was selected for chimera generation. c Southern blot data of DNA extracted from the litter of chimera and flipase mice. d PCR conditions for genotyping of miR-147 ^−/− mice. e Primer sequences are complementary to miR-147 allele. f Gel image from PCR-based genotyping to detect deletion of miR-147 allele.