Figure 6.

Upregulation of the ubiquitin-proteasome system in Ubqln2-Tg animals. A, GO-term enrichment analysis was performed on hippocampus (H) and lumbar spinal cord (SC) from Ubqln2^−/−, Ubqln2 KI (P520T), Ubqln2 WT transgenic, or Ubqln2 (P497S) mutant transgenic mice compared with their respective controls to identify pathways that were altered upon Ubqln2 perturbation, as in Figure 4. The most significantly enriched pathways were selected for visual summarization from Table S4. B, GO-term GO:0000502 Cellular Compartment (CC) proteasome complex breakdown of individual proteins in hippocampus from Tg animals (similar to Fig. 4B). C, western blot against ubiquitin (Ub) shows accumulation of ubiquitin conjugates (poly-Ub) in mutant Ubqln2-transgene (mutant Tg) expressing hippocampal tissues of mice. GAPDH is a loading control. BP, biological process; MF, molecular function.