Figure 2.

Passive transfer of GlyR‐IgG from a PERM patient affects forced walking ability. Behavioural testing of adult mice exposed to GlyR‐IgG, HC‐IgG or saline. (a) On day 14 when the behavioural testing had been finished, human IgG was found in both GlyR‐IgG and HC‐IgG‐injected mice with no difference between them (Two‐sided Student’s t‐test t = 1486 df = 8, P = 0.176). (b) Cell‐based assay showed that GlyR‐Abs were present in the GlyR‐IgG mice although not at high titre (arrows (orange) point to the merged GlyR‐Ab (red) bound to the surface of GlyR‐EGFP cells (green)). (c–g) Examples of some test results are shown. (c, d) In the open field, all three LPS groups showed a decrease in peripheral crosses compared with the non‐injected mice (One‐way ANOVA F = 9.810 df = 3 P < 0.0001). (e, f) In the dark/light boxes there was a suggestion of increased time in the light in the dark/light box in the GlyR‐IgG group but no significant difference from HC‐IgG‐injected mice. (g) The GlyR‐IgG mice took more time to turn around on the narrow beam before traversing the beam at baseline, but the four groups did not differ thereafter. (h, i) Mice injected with GlyR‐Ab i.p. showed a significant decrease in the time before falling off the rotarod compared to mice treated with HC IgG, saline or untreated mice. The effect was most clearly evident on day 10, and when compared to baselines the GlyR‐IgG‐treated mice spent significantly less time on the rotarod at days 5 and 10 of injection (*); (h) Two‐way ANOVA F = 17.24 df = 162 P < 0.0001 and (i) Summary of the data during the injected days One‐way ANOVA (F = 30.0 df = 23 P < 0.0001).