Figure 4.

IgG deposits were found in brain regions that express GlyRα1. Double labelled images from the PnC of the brainstem and ventral horn of the spinal cord. In GlyR‐IgG‐treated animals, human IgG (green) colocalizes with GlyRα1 antibody (red), and on close inspection some IgG is within the cytoplasm (asterisks (a, c)). In healthy control‐treated animals, the human IgG (green) is located only in the vessels and does not colocalize with GlyR (b, d). (e, g) Higher resolution images confirm that IgG deposits are localized intracellularly in both brainstem and spinal cord. In addition, there is human IgG bound on the surface, apparently separated from GlyRα1 (red). In HC‐IgG‐treated animals only vascular human IgG (green) is seen (f, h). Nuclei are stained with DAPI (blue). Confocal photographs were taken at 63× magnification and slightly cropped images are shown.