Table 2.
Results of the behavioural effects of PERM patient GlyR IgG
| Test | GlyR IgG Mean ± SE. n (6) | HC IgG Mean ± SE. n (6) | Saline Mean ± SE. n (6) | Non‐injected Mean ± SE. n (6) | P value* ANOVA Column factor | P value* GlyR v. HC Post hoc |
|---|---|---|---|---|---|---|
| Weight (g) | 24.44 ± 0.37 | 23.96 ± 0.56 | 24.39 ± 0.60 | 27.30 ± 0.51 | F (3, 134) = 56.14; P < 0.0001 | P = 0.70 |
| Open field, peripheral crosses (n) | 32.33 ± 5.90 | 30.00 ± 4.71 | 31.82 ± 4.04 | 43.11 ± 4.87 | F (3, 134) = 9.11; P < 0.0001 | P = 0.91 |
| Open field, total crosses (n) | 29.86 ± 6.19 | 32.21 ± 5.43 | 33.26 ± 4.35 | 47.87 ± 5.67 | F (3, 134) = 11.07; P < 0.0001 | P = 0.92 |
| Inverted mesh strength test, time (s) | 266.66 ± 11.36 | 252.11 ± 17.37 | 261.79 ± 15.84 | 258.93 ± 22.23 | F (3, 134) = 0.79; P = 0.50 | P = 0.68 |
| Wide runaway, time (s) | 13.49 ± 1.21 | 13.75 ± 1.66 | 13.39 ± 1.67 | 14.10 ± 1.71 | F (3, 134) = 0.25; P = 0.86 | P = 0.99 |
| Wide runaway, number of steps | 19.36 ± 0.69 | 19.82 ± 0.73 | 18.85 ± 0.79 | 19.15 ± 0.72 | F (3, 134) = 1.51; P = 0.22 | P = 0.78 |
| Light–dark compartment, crosses (n) | 3.40 ± 0.77 | 2.74 ± 0.59 | 2.50 ± 0.48 | 2.60 ± 0.40 | F (3, 134) = 2.01; P = 0.12 | P = 0.51 |
| Light–dark compartment, time in dark, (s) | 279.30 ± 9.55 | 285.31 ± 8.30 | 286.89 ± 6.13 | 287.50 ± 5.00 | F (3, 134) = 2.23; P = 0.09 | P = 0.36 |
| Light–dark compartment, time in light, (s) | 20.70 ± 9.55 | 14.69 ± 8.30 | 13.11 ± 6.13 | 12.50 ± 5.00 | F (3, 134) = 2.23; P = 0.09 | P = 0.36 |
| Successive alleys, time in closed alley (s) | 290.49 ± 2.75 | 293.38 ± 1.96 | 290.73 ± 3.42 | 288.75 ± 3.49 | F (3, 134) = 2.25; P = 0.09 | P = 0.46 |
| Successive alleys, time in open alleys time (s) | 9.10 ± 2.48 | 6.46 ± 1.97 | 8.89 ± 3.33 | 11.23 ± 3.21 | Kruskal–Wallis: H (4) = 5.45; P = 0.14 | Dunn’s P > 0.99 |
| Narrow Beam, time to turn (s) | 1.36 ± 0.21 | 1.57 ± 0.24 | 1.68 ± 0.44 | 1.95 ± 0.61 | F (3, 134) = 0.96; P = 0.41 | P = 0.99 |
| Narrow beam, total time to traverse (s) | 8.44 ± 0.79 | 9.93 ± 1.28 | 9.67 ± 1.41 | 10.18 ± 1.67 | F (3, 134) = 2.05; P = 0.11 | P = 0.27 |
| Narrow beam, number of steps | 18.88 ± 0.57 | 19.48 ± 0.76 | 18.92 ± 0.60 | 18.65 ± 0.65 | F (3, 134) = 1.72; P = 0.17 | P = 0.46 |
| Narrow beam, walking score | 7.13 ± 0.35 | 7.01 ± 0.50 | 7.20 ± 0.35 | 7.55 ± 0.26 | F (3, 134) = 2,24; P = 0.09 | P = 0.95 |
| Rotarod, time (s) | 149.45 ± 31.29 | 214.04 ± 34.75 | 212.51 ± 18.53 | 239.51 ± 15.82 | F (3, 134) = 17.39; P < 0.0001 | P = 0.0005 |
SE, Standard error, n, Number of mice, g, Grams, s, Seconds. *Two‐way ANOVA, Column factor (group), row factor (day), Post hoc analysis Tukey’s multiple comparison. Non‐significant if P > 0.05.