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. 2021 Jan 15;11:611122. doi: 10.3389/fmicb.2020.611122

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Copyright © 2021 Bharti, Maurya, Venugopal, Singh, Akhtar and Krishnan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Mycobacterium smegmatis expressing Rv1717 shows altered colony morphology and cell wall (CW) associated properties. (A) On MB 7H10 agar, M. smegmatis harboring empty vector (Msm^pMV261) formed rough colonies with irregular margins, while M. smegmatis expressing Rv1717 (Msm^Rv1717) formed smoother colonies with more regular margins. (B) Accumulation of EtBr and (C) Nile red by Msm^pMV261 and Msm^Rv1717 (D) Msm^Rv1717 and Msm^pMV261 were left untreated or treated with 0.05 or 0.1% (w/v) Sodium dodecyl sulfate (SDS) for 3 and 6 h. Viable cells in all samples were estimated by CFU assay. Statistical significance of data wherever applicable is indicated by ns: p > 0.05; ^***p < 0.001. Data plotted are mean ± SD of three independent experiments.